世界*實驗材料供應(yīng)商 Viagen Biotech, Inc.正式授權(quán)上海起發(fā)為其中國代理， Viagen Biotech, Inc.在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務(wù)，簽約 Viagen Biotech, Inc.就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲

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Viagen Biotech Inc.是創(chuàng)新的DNA提取技術(shù)和生命科學(xué)研究產(chǎn)品的供應(yīng)商。ViagenDirectPCR®DNA提取系統(tǒng)是一種單管系統(tǒng)，用于從小鼠尾巴，耳朵片，卵黃囊和培養(yǎng)細(xì)胞中快速制備DNA。由Viagen Biotech Inc.的科學(xué)家開發(fā)的正在申請的組件允許得到的DNA提取物與用于基因分型的基因組PCR相容。生物樣品的粗提取物與許多分子生物學(xué)級反應(yīng)（如聚合酶鏈反應(yīng)（PCR））不相容，部分原因是粗提物中含有抑制劑。DirectPCR試劑不僅介導(dǎo)生物樣品的快速溶解，而且還含有有效抑制粗裂解物對PCR擴(kuò)增的抑制活性的抑制劑，同時zui大限度地保持了釋放的基因組DNA的完整性。該過程*消除了任何溶液轉(zhuǎn)移或開管步驟，節(jié)省了大量的時間和精力。DirectPCR試劑每尾只花費26美分，可以節(jié)省任何使用量。

Viagenbiotech致力于簡便快速提取和制備核酸（DNA / RNA），以批量、快速進(jìn)行基因分析。公司擁有的技術(shù)不僅加快樣品的裂解，而且降低了粗提物中PCR抑制因素，因而使DNA提取與PCR基因分析兼容，樣品經(jīng)一步消化后即可進(jìn)行PCR擴(kuò)增，并且保證了基因組的完整性。

Proteinase K Solution 5ml (20mg/ml)

Availability: In stock

Quick Overview

Genomic PCR Grade Proteinase K Solution 

Catalog No. 501-PK

Stable for 12 months at -20℃ / Ships Refrigerated Fedex Overnight

Details

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.

DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.

Other applications: Proteinase K digests native proteins very effectively. It can therefore be used to remove DNases and RNases when isolating DNA and RNA from tissues or cells.

Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C

Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.

Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases

部分產(chǎn)品報價如下：

我們公司zui大優(yōu)勢是強(qiáng)大的采購，

1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，

2：貨品全，現(xiàn)經(jīng)營過700多個品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，

3：提供加急服務(wù)，一般1-2周到貨，超過時限加急費全免

4：價格公道，絕大部分價格有優(yōu)勢，當(dāng)然不能保證100%產(chǎn)品都是,因為意味著沒有服務(wù).

5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大 交大 復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥 ，fisher等500多家公司

6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc.  Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等

7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā)，歡迎合作。