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5´ AOX1 promoter region: bases 1-941
5´ AOX1 priming site: bases 855-875
α-factor signal sequence: bases 941-1207
Multiple cloning site: bases 1208-1276
c-myc epitope: bases 1275-1304
Polyhistidine （6xHis） tag: bases 1320-1337
3´ AOX1 priming site: bases 1423-1443
AOX1 transcription termination region: bases 1341-1682
TEF1 promoter: bases 1683-2093
EM7 promoter: bases 2095-2162
Sh ble ORF: bases 2163-2537
CYC1 transcription termination region: bases 2538-2855
pUC origin: bases 2866-3539 （complementary strand）
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